Journal: Brain, behavior, and immunity

Article Title: Innately activated TLR4 signal in the nucleus accumbens is sustained by CRF amplification loop and regulates impulsivity

doi: 10.1016/j.bbi.2017.11.008

Figure Lengend Snippet: Percentage of co-staining cells

Article Snippet: Other antibodies were goat anti-GAD67 (glutamic acid decarboxylase 1 (GAD1)) (LifeSpan BioSciences, Seattle, WA, USA, Cat. # LS-B3027-50, RRID: AB_1965223), rabbit anti-CRFR1 (Thermo Fisher Scientific, Waltham, MA, USA, Cat. # 720290, RRID: AB_2633242), rabbit anti-NF-kB p65 (Abcam, Cambridge, MA, USA, Cat. # ab32536, RRID: AB_776751), mouse beta Actin (β-Actin; Proteintech Group, Rosemont, IL, USA, Cat. # 66009-1-Ig, RRID: AB_2687938), Alexa Fluor 488 goat anti-rabbit or donkey anti-goat IgG (H+L; Cat. # {"type":"entrez-nucleotide","attrs":{"text":"A11034","term_id":"489250","term_text":"A11034"}} A11034 , RRID: AB_2576217 or Cat. # {"type":"entrez-nucleotide","attrs":{"text":"A11055","term_id":"490909","term_text":"A11055"}} A11055 , RRID: AB_142672, respectively, Thermo Fisher Scientific), and Alexa Fluor 546 goat anti-mouse or goat anti-rabbit IgG (H+L; Cat.# {"type":"entrez-nucleotide","attrs":{"text":"A11030","term_id":"489248","term_text":"A11030"}} A11030 , RRID: AB_2534089, or Cat. # A11035, RRID: AB_2534093, respectively, Thermo Fisher Scientific).

Techniques: Expressing

Journal: Nature neuroscience

Article Title: An inhibitory hippocampal–thalamic pathway modulates remote memory retrieval

doi: 10.1038/s41593-021-00819-3

Figure Lengend Snippet: Representative images showing immunohistochemical staining in ADn of WT home cage mice with a, inhibitory cell marker GAD67 (repeated in n = 3), or b, excitatory cell marker aCamKII (repeated in n = 3; scale bar: 100 μm).

Article Snippet: Sections were washed with PBS (three times), then incubated with PBS containing goat anti-rabbit ALEXA Fluor 633 (for CTB, 1:500 dilution; Thermo Fisher Scientific Cat. no. A-21070 RRID:AB_2535731) and goat anti-mouse ALEXA Fluor 488 (for GAD67, 1:500 dilution; Thermo Fisher Scientific Cat. no. A-11001 RRID:AB_2534069) secondary antibodies for 24 h at 4 °C.

Techniques: Immunohistochemical staining, Staining, Marker

Journal: Nature neuroscience

Article Title: An inhibitory hippocampal–thalamic pathway modulates remote memory retrieval

doi: 10.1038/s41593-021-00819-3

Figure Lengend Snippet: a,b, WT mice were micro-infused with CTB (n = 3) in the ADn, and retrograde-labeled neurons in CA3 were visualized. Sections were costained with the inhibitory cell marker GAD67. Numerous CTB+ and GAD67+ colocalized cells were visualized in CA3 (white arrows). c,d, To identify inhibitory projections to the ADn, VGAT-Cre mice were micro-infused with AAV-DIO-EYFP in CA3 (n = 3). Strong terminal projections were visualized in the ADn. e–g, To visualize specifically CA3–ADn projections and examine whether they are inhibitory, WT mice were micro-infused with AAV-DIO-mCherry bilaterally in CA3 and AAVrg-Cre-EGFP unilaterally in ADn, and sections were costained with (f) GAD67 (CA3) or (g) VGAT (ADn) (n = 4). Numerous inhibitory CA3–ADn projection neurons in CA3 (mCherry+ and GAD67+, white arrows) and projections in ADn (mCherry+ and VGAT+, white arrows) were visualized (blue scale bars, 100 μm; white scale bars, 50 μm).

Article Snippet: Sections were washed with PBS (three times), then incubated with PBS containing goat anti-rabbit ALEXA Fluor 633 (for CTB, 1:500 dilution; Thermo Fisher Scientific Cat. no. A-21070 RRID:AB_2535731) and goat anti-mouse ALEXA Fluor 488 (for GAD67, 1:500 dilution; Thermo Fisher Scientific Cat. no. A-11001 RRID:AB_2534069) secondary antibodies for 24 h at 4 °C.

Techniques: Labeling, Marker

Journal: eNeuro

Article Title: Transient Seizure Clusters and Epileptiform Activity Following Widespread Bilateral Hippocampal Interneuron Ablation

doi: 10.1523/ENEURO.0317-23.2024

Figure Lengend Snippet: A , Plasmid map for the FlpO-dependent (frt) mCherry reporter. B , Plasmid map for the frt-DTr expression vector. C , Plasmid map for the frt-hM4D i /HA (DREADD) expression vector. D , The specificity of Vgat-FlpO mice was tested by injecting animals with AAV9-frt-mCherry (red), immunostaining hippocampi with the GABAergic marker Gad67 (green), and counterstaining with DAPI (blue). mCherry and Gad67 are extensively colocalized in the dentate gyrus, with little to no labeling of excitatory granule cells. Scale bar, 200 µm. E , High resolution images show colocalization of mCherry in Gad67-immunoreactive hilar interneurons. Scale bar, 25 µm.

Article Snippet: Brain sections from study animals were immunostained using the following antibodies: DTr (1:500 goat anti-HB EGF, #AF259SP, R&D Systems and 1:750 donkey anti-goat Alexa Fluor 568, #A11057, Thermo Fisher Scientific), GAD1/GAD67 (1:500 chicken anti-GAD1/GAD67, #198006, Synaptic Systems and 1:750 goat anti-chicken Alexa Fluor 488, #A11039, Thermo Fisher Scientific), somatostatin (1:500 rabbit anti-somatostatin, #PA5-82678, Invitrogen and 1:750 donkey anti-rabbit Alexa Fluor 647, #A-31573, Invitrogen), parvalbumin (1:1,000 guinea pig anti-parvalbumin, #195004, Synaptic Systems and 1:750 donkey anti-guinea pig DyLight 405, #706475148 Jackson ImmunoResearch or Alexa Fluor 647, #706605148, Jackson ImmunoResearch), neuropeptide Y (1:300 rabbit anti-neuropeptide Y, #N9528, Sigma-Aldrich and 1:750 donkey anti-rabbit Alexa Fluor 488, #R37118, Thermo Fisher Scientific or 1:750 goat anti-rabbit Alexa Fluor 488, #A11008, Thermo Fisher Scientific), hemagglutinin (1:500 rat anti-HA, #11867423001, Roche and 1:750 goat anti-rat Alexa Fluor 568, #A11077, Thermo Fisher Scientific), and gephyrin (1:1,000 chicken anti-gephyrin, #147009, Synaptic Systems and 1:750 donkey anti-chicken Alexa Fluor 488, #A78948, Invitrogen).

Techniques: Plasmid Preparation, Expressing, Immunostaining, Marker, Labeling